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关于摘要翻译的问题!

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Purpose: the prophase work found that bitter melon protein M28 can raise THP 1 source sex foam cell ABCA1 expression and reduce its intracellular lipid accumulation, but its mechanism is not clear.

关于摘要翻译的问题!

Methods: qRT PCR and western blot detection respectively ABCA1 mRNA and protein expression level, liquid scintillation counting method for determination of cholesterol, oil red O staining method determination of intracellular lipid accumulation, statistical analysis using mean + / - standard deviation (+ / - SD) said that using Graphpad Prism5.0.1 to analyze data and drawing, 95% confidence interval, P < 0.05 for the difference has a significant meaning. Oil red O staining test bubble lipids in the cell, high performance liquid chromatography (HPLC) to detect intracellular cholesterol fat and cholesterol. Western blot detection THP - 1 source sex protein expression in foam cells

Results: the expression of balsam pear cut ABCA1 protein MD28, bitter melon protein MD28 dose (0 mg/ml, 0.4 mg/ml, 1.2 mg/ml, 3.6 mg